FastQCFastQC Report
Wed 25 May 2016
SRR1700270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197877
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109710.11927752458529288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG107150.027.6576772
GTACCTG110300.027.426431
ACCTGGG115800.025.0272163
GGTATCA90700.023.3049561
GTATCAA178100.021.3808291
CCTGGGG82250.020.72384
GTACCCG8100.019.2167551
GTACCGG9600.017.6240631
GTACACG8500.017.5162451
ATAACGC7300.017.2745883
ATCAACG218850.016.9262943
TCAACGC219850.016.8070534
CAACGCA224100.016.5205925
TATCAAC231100.016.1366122
TATAACG6700.016.1331232
AACGCAG233250.016.094186
TACCGGG9100.016.0851082
TACCCGG9350.015.6550242
TAACGCA7500.015.31152254
CTGGGGG45000.014.3539325
Produced by FastQC (version 0.11.5)