FastQCFastQC Report
Wed 25 May 2016
SRR1700269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7258175
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109130.15035460015775318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90840.1251554281895931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93100.024.9102231
GTACCTG63200.022.751791
TACCTGG62750.022.5043322
GTATCAA173100.020.090041
ACCTGGG69700.019.9689873
TATAACG3600.016.2660882
ATCAACG218100.015.6443413
TCAACGC220450.015.4977914
CCTGGGG50250.015.4175964
TATCAAC225550.015.2678172
CAACGCA224850.015.2074755
AACGCAG233000.014.8205686
ATAACGC4650.014.5300993
TAACGCA5050.014.2709514
TATACCG3650.014.1755975
CCTATCG1000.007563829413.4979128
CCGTTAG1352.6589306E-413.33099637
GTATTAG20950.013.232231
TCTATAC17800.013.1587213
ACGCAGA266150.012.9239837
Produced by FastQC (version 0.11.5)