FastQCFastQC Report
Wed 25 May 2016
SRR1700268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027722
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159980.15953772950626274No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA147000.14659361318552708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123530.1231884968490351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG66900.017.3847711
TACCTGG65050.017.17472
GTATCAA306550.017.1243291
GGTATCA190600.016.9105831
ACCTGGG71450.015.1315353
CGCGTAA950.005385911614.20811310
ATCAACG368250.013.9700283
TCAACGC369400.013.920374
TATCAAC375050.013.8131732
CAACGCA374450.013.7155165
ATAACGC5750.013.7102123
AACGCAG381500.013.5211586
TCTATAC24700.013.4961133
TATAACG6050.013.4029972
TAACGCA6250.013.3340934
GAGTACT239950.013.18175812
AGAGTAC364600.013.13613911
GTACATG250550.013.0262571
GTATACG4050.012.8002431
TATACTG37700.012.7097895
Produced by FastQC (version 0.11.5)