FastQCFastQC Report
Wed 25 May 2016
SRR1700267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5729011
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100660.17570222853473313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82080.14327080188884259No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA78310.13669025945315866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.10102965415845773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG64450.026.3842642
GTACCTG66450.026.3203351
ACCTGGG69450.023.8679523
TATAACG5550.021.5081922
TAACGCA5450.021.0755774
CCTGGGG53750.020.238324
ATAACGC5850.020.0196783
GTACACG6150.019.4234091
TACACGG5850.018.0951822
GTATCAA171700.017.0646631
GGTATCA107350.016.8172571
CGCGTAT850.002569590715.87980212
CGCGGTA2850.015.787765543
TATACTG18900.014.7592765
GACCGTC2001.5807018E-814.622787
CTGGGGG29150.014.5085675
GTACCCG6000.014.2743731
GTATAAC12300.014.1094571
TTAACGG3350.014.10184435
TACCCGG5850.013.860142
Produced by FastQC (version 0.11.5)