FastQCFastQC Report
Wed 25 May 2016
SRR1700266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8010447
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116850.14587200938973818No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA85780.10708516016646763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84140.10503783371889235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG79050.023.6796282
GTACCTG81750.023.3290881
ACCTGGG82800.022.0626743
TATAACG5800.019.8069632
ATAACGC7100.017.4490553
GTATCAA219450.016.4361291
TAACGCA6950.016.205044
CCTGGGG69850.015.7368674
GGTATCA141050.015.4709661
GTACATG203150.013.7933411
CCGGTCG4450.013.6491989
TACATGG201850.013.2798742
ATCAACG265800.013.1778023
TCAACGC267400.013.0904474
CGGTCGT3450.013.041100510
TATCAAC271100.012.9951992
GTATTAG22950.012.9660121
CAACGCA270900.012.9623485
ACGTCCG3500.012.85536143
AACGCAG277250.012.7305356
Produced by FastQC (version 0.11.5)