FastQCFastQC Report
Wed 25 May 2016
SRR1700265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7674578
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA161510.21044805330012933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122950.16020424836388397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91790.11960266740399277No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA77940.10155607252932995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG62850.022.0230871
TACCTGG62400.021.443972
ATAACGC5900.021.380583
TATAACG5150.020.9960352
ACCTGGG66900.019.3272153
TCGCACG1507.3032425E-917.99623718
TAACGCA6000.017.6452264
CGCGGTA3850.016.9453943
GTATCAA271050.014.8957131
CCTGGGG51150.014.6208664
CGGTACC4350.014.51481345
TAGACCG1553.6312376E-614.5131895
GTACATG177650.014.0728291
CGATCGC1951.8381252E-713.8432615
GTACCCG6100.013.6737291
GTACACG6150.013.5625621
GTACCGG5850.013.48737051
TACCCGG6200.013.443532
CGAACTA5200.013.41091929
TACATGG175500.013.4006762
Produced by FastQC (version 0.11.5)