FastQCFastQC Report
Wed 25 May 2016
SRR1700264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6850087
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA671030.9795933978648739No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA243510.3554845361818032No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT198830.2902590872203521No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT176030.25697483842176017No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC166190.24261005736131527No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA146380.2136907166288545No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT128150.1870779159447172No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA121070.17674228079147025No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT103500.15109297151992376No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT99110.1446842937907212No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG96680.14113689358981865No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA81440.11888900097181247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77960.11380877352360635No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT77170.11265550349944461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG69800.024.1540161
TACCTGG69850.023.8602582
ACCTGGG75850.021.4375723
GTACACG6450.016.773541
CCTGGGG60150.016.5118964
CGCGTAA850.002569319715.88011610
TACACGG6750.015.3484282
GTACCCG6050.014.9021131
CGCGAAT1108.605648E-414.31606228
TATAACG4500.014.0137822
TACCCGG5850.013.8597842
GAGTACG9350.013.4995591
ATAACGC5400.013.3459723
AGTACGC7350.013.1762232
CGAACGT1555.766537E-513.07694
GTACATG153500.012.9216351
ACAACGC4900.012.86932853
TCTATAC14900.012.8477373
TACATGG149350.012.7276152
TATACTG21900.012.6347475
Produced by FastQC (version 0.11.5)