FastQCFastQC Report
Wed 25 May 2016
SRR1700263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165249
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA214770.41579796056298546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69830.13519193363185394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.1293645282153871No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51820.10032430188747919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG51850.024.0898651
TACCTGG51100.023.9304662
ACCTGGG53850.022.163873
TATAACG4350.019.1551132
TAACGCA4650.017.4339794
CCTGGGG43500.017.2385984
ATAACGC4450.017.2057783
GTACCGG4900.017.0246811
TACCGGG4650.015.9820782
GTCGTAC1502.5233549E-615.0308011
CGCGTAA900.003759760214.99791610
CGCGTAT1150.00120438813.69321912
GTACCCG4350.013.4758891
CCGACGC1851.5333317E-613.3762632
GTATAAC10350.013.2880991
TACACGG4250.013.2471762
TCTATAC11250.012.8109943
TACCCGG4550.012.3737362
GGTCTAG5300.012.33661
TAGACTG12200.012.1769235
Produced by FastQC (version 0.11.5)