FastQCFastQC Report
Wed 25 May 2016
SRR1700262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680534
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA521321.1138045359781599No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA217930.46560926595127816No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT162580.34735352846491446No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT141730.30280732924918397No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC130750.27934846750392156No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA114440.24450201622293524No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT97130.2075190565862784No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA84790.1811545434773041No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG72100.1540422524438451No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT71890.15359358568915427No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT70800.1512647915814734No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA63540.13575374091930537No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT62290.13308310547471722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58610.12522075472584965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56750.030.175911
TACCTGG56500.029.8946192
ACCTGGG61350.027.0905233
TATAACG3750.022.220342
TAACGCA3550.022.202714
CCTGGGG43250.021.9210994
ATAACGC3950.020.5248973
AGTACGC3650.019.1270962
GTACACG3600.018.1752341
GTACCGG3800.017.8123871
CTGGGGG22000.017.699365
GTCGTAC1953.274181E-1117.3556611
TACGCAG4200.016.6218244
GAGTACG5250.016.3308491
GTATAAC8950.015.629791
TCACGCA3950.015.3933434
GTACCCG4550.015.3721551
GTACGCA4650.015.0135813
TACCCGG4600.014.6873582
CGCCGTA1705.912625E-714.55581725
Produced by FastQC (version 0.11.5)