FastQCFastQC Report
Wed 25 May 2016
SRR1700261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392252
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56400.12840793287816818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.12230627933005665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG41600.022.4615692
GGTATCA69250.022.3376961
GTACCTG42900.021.9185981
ACCTGGG46150.019.8567453
ATAACGC3200.018.2940033
TAACGCA3250.018.0117344
TATAACG3550.016.4903682
GTATCAA108000.015.9724561
CCTGGGG40000.015.0848284
TAGACCG950.005370592714.2139625
TATACTG14000.013.50326255
CCGTTAA2053.3768993E-713.16851616
GTACACG3500.012.8853431
TACCCGG4150.012.4785782
ATCAACG134800.012.427063
GCTATAC5800.012.4224523
TCAACGC135950.012.3876224
CGTATAC1852.2303384E-512.1706463
TCTATAC9450.012.1513283
TACACTG19650.012.1403385
Produced by FastQC (version 0.11.5)