FastQCFastQC Report
Wed 25 May 2016
SRR1700260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7235325
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119140.16466433781481826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90770.12545393607059807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG78250.025.9189551
TACCTGG76800.025.6597372
GGTATCA83650.024.1109161
ACCTGGG84350.023.095023
ATAACGC5850.021.5585483
GTATCAA160500.021.0702061
TAACGCA5250.020.5889054
TATAACG5650.020.329522
CCTGGGG61400.019.2916184
GTACACG5950.018.5789551
ATCAACG202250.016.4244523
GTACCGG6650.016.2840251
TCAACGC205350.016.1751614
CAACGCA209550.015.8531555
TATCAAC211700.015.8515162
TACCGGG6150.015.7470322
AACGCAG217450.015.3699366
TACACGG6600.015.3558542
ACGTCGC1901.3384306E-714.20765922
ACGCAGA247100.013.5105817
Produced by FastQC (version 0.11.5)