FastQCFastQC Report
Wed 25 May 2016
SRR1700259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232742
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.12370303599854242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.1207952877155059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG35150.024.4536721
GGTATCA41600.024.4041461
TACCTGG35150.024.0257192
ATAACGC3700.021.9111143
ACCTGGG37550.021.8900453
TATAACG3400.021.857752
TACACGG3250.021.4807152
TAACGCA3700.020.693834
GTACACG3500.019.981961
GTATCAA70650.018.0418361
TACGCGC1002.0425901E-518.0060486
CCTGGGG27900.017.1117884
GTATAAC7500.015.9425961
TACCCGG3050.014.76728252
GTACCGG2601.0913936E-1114.7509511
ACACGGG4550.014.35325053
GTACGCG1108.570447E-414.3234375
ATCAACG90450.013.86788753
TCAACGC91200.013.7538434
TATCAAC94550.013.5763722
Produced by FastQC (version 0.11.5)