FastQCFastQC Report
Wed 25 May 2016
SRR1700258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582655
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86810.15549948904240007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71650.12834395104121607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58250.025.9054151
TACCTGG57400.025.8521922
GGTATCA63900.023.8619651
ACCTGGG64350.022.4648023
GTATCAA123450.020.8658161
ATAACGC4450.019.2282473
GTACACG5100.018.5754571
TATAACG4700.018.2057932
GTACCCG5500.018.044731
TAACGCA4950.017.7402574
CCTGGGG49300.017.7208824
TACACGG5700.016.987032
TACCCGG5600.016.4861682
ATCAACG157750.016.1439323
TCAACGC160200.015.8964664
TATCAAC163100.015.7113032
CAACGCA164650.015.5003575
AACGCAG170800.015.1401546
ACACGGG6400.015.1288023
GTACCGG4800.015.0372731
Produced by FastQC (version 0.11.5)