FastQCFastQC Report
Wed 25 May 2016
SRR1700257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7686966
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107380.1396910042271554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83170.1081961335590661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG118400.030.9815481
TACCTGG118200.030.5824362
ACCTGGG127500.027.6975823
TATAACG7500.025.2250792
CCTGGGG90050.024.3102024
GGTATCA83400.024.086791
ATAACGC7900.023.6623043
TAACGCA8600.021.7363014
GTATCAA152750.020.6787471
GTACCCG10100.018.7510341
CTGGGGG47450.017.3502355
GTATAAC16450.016.8580171
CGCACGA4450.016.68070612
TACCCGG10700.016.6286812
GTACCGG7750.016.5821341
ATCAACG187950.016.224913
TCAACGC188700.016.196234
CAACGCA193850.015.7461555
TATCAAC196700.015.6752192
ACAACGC4000.015.2023173
Produced by FastQC (version 0.11.5)