FastQCFastQC Report
Wed 25 May 2016
SRR1700256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10236314
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154000.15044477924377855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118640.1159010948667655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG115450.027.7278121
TACCTGG114350.027.4575022
GGTATCA102750.026.3062231
ACCTGGG124800.024.6526813
GTATCAA207850.021.8873061
TATAACG8150.021.2797992
TAACGCA8700.019.416464
ATAACGC9750.019.1735063
CCTGGGG92150.019.162354
ATCAACG258450.017.2550683
TCAACGC261800.017.0428754
CAACGCA267450.016.7279555
TATCAAC269950.016.6787642
GTACCCG11250.016.2313141
AACGCAG278250.016.1839246
TACCCGG11000.015.9711542
GTACCGG10150.014.6588221
GACGCTA5950.014.36538640
ACGCAGA322100.013.95274457
CTGGGGG49600.013.9221835
Produced by FastQC (version 0.11.5)