FastQCFastQC Report
Wed 25 May 2016
SRR1700255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5623362
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73890.13139826317423633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.10468826299996337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG84700.031.2784791
TACCTGG84600.031.0698412
ACCTGGG91700.027.9263523
TAACGCA5800.024.8527854
TATAACG5350.023.9971492
CCTGGGG58700.022.906534
ATAACGC6050.022.7089773
TACCCGG6050.021.592912
GTACCCG6200.021.092471
GTACCGG6000.019.9166281
GTACACG5300.019.1437851
GTATCAA139350.017.7820531
GGTATCA82250.017.3797911
GTCTCGC8300.017.1140841
CTGGGGG29750.016.785145
TACCGGG6250.016.5773982
TACACGG5750.016.452052
CGACCGT1703.4244295E-815.87825821
TCTGTCG9900.015.4501498
TCGCCGA1908.047209E-915.39035717
Produced by FastQC (version 0.11.5)