FastQCFastQC Report
Wed 25 May 2016
SRR1700254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17243687
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA433300.2512803671279814No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA212520.12324510413579184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA11150.017.95454843
GTACCTG97500.017.3208471
GCGAACT18100.017.02674328
CGAACTA17550.016.91945529
TACCTGG100050.016.389652
GTACATG470900.014.7617141
GTACCGG7200.014.4051331
CGGTACC14450.014.40334845
TAACGGC14200.014.25672536
TACATGG455650.014.0787472
GTATTAG60550.013.7033141
ACATGGG455100.013.5358553
TTAACGG15650.013.51077835
GTATCAA581000.013.47396851
GCCGTTA13700.013.46329515
ACCTGGG115900.013.4480053
TACCGCG13250.013.4113298
AACGGCC15350.013.33505637
ACGGCCG15400.013.29164538
ATACCGC12100.013.1987737
Produced by FastQC (version 0.11.5)