FastQCFastQC Report
Wed 25 May 2016
SRR1700253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012964
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121060.15108017457709783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89430.11160664143755046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG129100.030.4215931
TACCTGG127550.030.1978552
ACCTGGG129000.029.1255513
GTACCCG10150.025.7219981
TATAACG7800.025.6713222
CCTGGGG93600.023.6989734
TAACGCA9000.022.9971374
TACCCGG11150.022.8011342
GGTATCA91750.022.1019951
ATAACGC9800.022.0390343
GTATCAA177250.021.154441
CGCGTAT1403.101377E-919.28271112
GTACACG8300.018.9815961
GTACCGG7850.018.6361621
TACACGG8350.018.32212
CTGGGGG49650.018.0787545
ATCAACG212750.017.3217493
TCAACGC214450.017.2151574
CAACGCA219000.016.9596865
TATCAAC222500.016.5629142
Produced by FastQC (version 0.11.5)