FastQCFastQC Report
Wed 25 May 2016
SRR1700252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9926852
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130620.1315824996685757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG122100.025.766151
TACCTGG119550.025.744932
GGTATCA106250.025.3102841
ACCTGGG126050.023.7565773
GTATCAA211000.021.8959871
TACCCGG11650.020.2776952
GTACCCG13050.018.9688151
CGCGTAA1102.262832E-618.40626510
CCTGGGG93900.018.1371064
TATAACG7100.018.0622442
ATCAACG254000.017.7860153
TCAACGC256400.017.6278614
TAACGCA7450.017.514974
CAACGCA261050.017.347465
TATCAAC265400.017.1240162
AACGCAG273000.016.7776416
ATAACGC7650.016.7633973
GTACACG9000.016.2528271
CGCGCAA1803.929017E-916.24766721
GTACATG265700.014.5254831
Produced by FastQC (version 0.11.5)