FastQCFastQC Report
Wed 25 May 2016
SRR1700251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6304668
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73760.11699267907525027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG101400.030.2747921
TACCTGG102400.029.6395782
ACCTGGG105700.028.1811833
TAACGCA6700.026.1910114
TATAACG6400.025.6627332
ATAACGC7000.024.426533
CCTGGGG68550.023.8922144
GTACCCG8500.021.447211
TACCCGG9100.020.5209162
GTATCAA137200.019.7012421
GGTATCA76050.019.6504971
GTACCGG6500.019.0438181
GTACACG5500.018.414271
CTGGGGG35200.016.169375
TACACGG6150.016.0967272
ATCAACG164300.016.0211813
GTATAAC15200.015.8432731
TCAACGC166550.015.8312594
TACCGGG6750.015.6658542
CAACGCA169650.015.5546195
Produced by FastQC (version 0.11.5)