FastQCFastQC Report
Wed 25 May 2016
SRR1700250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8850061
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG134300.029.8639011
TACCTGG133650.029.4260962
ACCTGGG140150.027.5149123
CCTGGGG112500.022.0372494
TATAACG5700.021.7094122
TAACGCA7600.019.241024
ATAACGC7150.019.1943843
GTACACG9050.018.9007851
GTACCCG12550.018.4717641
GTATCAA185900.018.2330951
TACACGG9450.017.380062
GGTATCA113750.016.97661
TACCCGG12900.016.3945062
CTGGGGG57850.015.4770575
GTACCGG8550.015.0045711
GTATAAC17400.014.8752221
ATCAACG221200.014.8598863
TCAACGC222450.014.7655214
CAACGCA225600.014.638475
TATCAAC232500.014.244432
Produced by FastQC (version 0.11.5)