FastQCFastQC Report
Wed 25 May 2016
SRR1700249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11002817
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA288820.26249641341849095No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA134140.12191423341858726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA13200.016.877729
CGCGGTA11350.016.25352743
GCGAACT13650.016.15628428
GTACCTG57350.015.6204821
TAACGGC12250.015.24331836
TACCTGG58400.015.1015172
CGTCCGG13450.015.05392944
ACGTCCG13300.014.88540543
TATACCG9700.014.6111576
GTACATG327450.014.5794281
CGGTACC12550.014.57066745
GTACCGG4800.014.0677551
TACATGG316300.013.9982362
CCGGTCG14650.013.9739639
GTATTAG45400.013.8322591
CGGTCGT14150.013.83179810
AACGGCC13800.013.69423337
TTAACGG13500.013.5003835
ATACCGC10000.013.4979277
GCGGTAC13900.013.27176544
Produced by FastQC (version 0.11.5)