FastQCFastQC Report
Wed 25 May 2016
SRR1700248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8007897
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112580.14058622382380792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85250.10645741322596933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG101500.029.114641
TACCTGG103650.028.2795332
ACCTGGG109900.026.0981433
TAACGCA6550.019.9197464
CCTGGGG81650.019.8644354
TATAACG6900.018.9093272
ATAACGC7300.018.1813563
GGTATCA126800.017.9739361
GTACCGG8150.017.7551461
TACACGG7950.017.5437182
GTACCCG8950.017.17861
GTATCAA207750.016.9888421
TACCGGG7300.016.6405642
TACCCGG9100.016.5626772
GTACACG9050.015.489771
CTGGGGG45650.014.2907285
GTATAAC15800.014.16706851
ATCAACG244050.013.9831133
GTCTCGC10350.013.9811051
TCAACGC246750.013.8574574
Produced by FastQC (version 0.11.5)