FastQCFastQC Report
Wed 25 May 2016
SRR1700247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13187794
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162150.12295460484141624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145150.024.8592471
GTATCAA292450.021.3445431
GTACCTG84250.018.1404841
TACCTGG83800.017.74432
ATCAACG352900.017.3260363
TCAACGC356900.017.1318534
CAACGCA363050.016.8602315
GTACCGG8750.016.7948991
TATCAAC371900.016.6465282
CGAACTA13900.016.51152629
AACGCAG374750.016.3818656
GCGAACT14300.016.3605828
GTACATG409650.014.9840421
CGCGGTA11300.014.73299243
ACCTGGG98750.014.5339963
TACATGG402050.014.3742592
CCGGTCG11950.013.9313529
ACGCAGA443550.013.7808667
GTACCCG8050.013.7617071
TACCGGG8350.013.7399492
Produced by FastQC (version 0.11.5)