FastQCFastQC Report
Wed 25 May 2016
SRR1700246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5524428
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.12222079824372768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG110700.032.47481
TACCTGG110000.032.2712942
GTACCCG10200.030.5897481
TATAACG5850.029.6098982
ACCTGGG118950.029.029933
GTACCGG6950.028.9535751
TAACGCA6200.028.3012084
TACCCGG10900.027.8618092
ATAACGC7250.026.9949993
TACCGGG7050.026.8035452
CCTGGGG63550.024.602054
GGTATCA61150.022.5543521
TACACGG4850.022.2639182
CGACCGT3400.021.83517621
GTACACG5250.020.671831
GTATAAC11450.019.5490971
TCGCCGA3950.019.36437617
GTATCAA111900.019.2961331
CTGGGGG27850.018.659025
GACGCTA9150.018.44121240
Produced by FastQC (version 0.11.5)