FastQCFastQC Report
Wed 25 May 2016
SRR1700245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7702301
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG135850.031.949141
TACCTGG139000.030.8311372
ACCTGGG148350.028.311713
TATAACG6800.025.1428222
TAACGCA7400.024.0162224
CCTGGGG99750.023.8155354
ATAACGC7550.023.5390823
TACCCGG11600.020.362952
GTACCCG12200.020.196881
TACACGG8350.019.936762
GTACACG8700.019.7474921
GTACCGG8400.019.6454141
CTGGGGG51450.018.0581825
GTATCAA147850.017.8735491
TACCGGG8400.016.3365332
GGTATCA90500.016.2860811
ACACGGG10100.015.8141663
CGCGTAT1004.1758252E-415.74755112
GTATAAC15400.015.7065451
ATCAACG171050.014.7563553
Produced by FastQC (version 0.11.5)