FastQCFastQC Report
Wed 25 May 2016
SRR1700244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8534908
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA224330.26283821688529035No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA109090.12781625765620439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106220.12445359692219295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG103900.027.616231
TACCTGG103800.027.0468332
ACCTGGG108100.025.2842313
TAACGCA6200.020.3186824
TATAACG6700.019.1381172
ATAACGC7100.018.6935943
CCTGGGG85800.018.2482454
TACCGGG7150.016.3604972
GTACCGG7800.016.2334841
GTACCCG10350.015.9478011
TACCCGG9800.015.8387982
GTACACG8650.015.1610811
GTACATG196000.014.212581
TACACGG8400.014.1937042
ACCGTCC4100.013.7178448
TACATGG194350.013.7161742
CGGTCGA1656.930557E-613.63470610
GTATAGG17300.013.2005971
GTATCAA288050.013.0853711
TACCGTC3800.013.0246567
Produced by FastQC (version 0.11.5)