FastQCFastQC Report
Wed 25 May 2016
SRR1700243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11019934
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116820.10600789442114626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG150100.028.546951
TACCTGG148650.028.541682
ACCTGGG154350.026.6131843
CCTGGGG113750.020.567654
GGTATCA120700.019.67041
TATAACG8450.019.1680742
GTATCAA212400.019.0239731
TAACGCA9000.018.996514
ATAACGC10050.018.8025153
GTACCGG11700.018.5531021
GTACCCG13350.017.7843991
TACCCGG13600.016.87192
GTACACG10050.015.97435951
TACCGGG11250.015.397172
ATCAACG257850.015.1979063
TCAACGC259250.015.1158334
CAACGCA264500.014.8498225
GTACATG249200.014.6358351
TCTGTCG15650.014.5187588
TATCAAC272200.014.4958682
Produced by FastQC (version 0.11.5)