FastQCFastQC Report
Wed 25 May 2016
SRR1700242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607916
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA149180.17330559452485364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115310.1339580915984775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87210.10131372099820676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG88150.028.1065391
TACCTGG90300.026.9064452
ACCTGGG98750.023.7839323
GTACCGG6200.022.2411541
GTACCCG7000.021.6369441
TACCCGG7150.020.4516142
TATAACG5900.019.4463652
TACCGGG6550.019.2338872
TAACGCA6550.017.5167794
CCTGGGG63800.017.489864
ATAACGC8300.015.9916843
GTACACG7300.015.7930681
TACACGG6200.015.6026032
GACGCTA8650.015.344840
GTATCAA264800.015.1103471
CGCGGTA4050.014.99828743
GTACATG224950.014.7120861
ACGCTAC9050.014.16924141
TACATGG221100.014.16350652
GGTATCA174250.014.0110321
Produced by FastQC (version 0.11.5)