FastQCFastQC Report
Wed 25 May 2016
SRR1700241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9552153
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123600.12939491233023592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG131000.028.4416351
TACCTGG130800.028.0008282
ACCTGGG137000.026.1588973
GTACCCG10350.022.499121
CCTGGGG96300.020.4645424
TATAACG7200.020.3097722
TACCCGG11400.020.1288782
GGTATCA123850.019.8062571
GTATCAA217800.019.110171
TAACGCA6850.019.0485384
ATAACGC8500.018.5269243
GTACCGG10150.018.4876081
GTACACG7650.016.5498941
TACCGGG10400.016.2237832
ATCAACG263600.015.4304023
TCAACGC265350.015.3540734
CTGGGGG47150.015.1729365
CAACGCA270450.015.0728525
TATCAAC274200.014.9405552
GTACATG253200.014.8936451
Produced by FastQC (version 0.11.5)