FastQCFastQC Report
Wed 25 May 2016
SRR1700240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12573656
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA1687211.3418611102451028No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA797250.6340637917881641No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT549950.4373827310052064No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT537610.4275685608068171No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC450810.35853533769334867No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA357710.28449163870874156No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT349180.2777076134419456No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA325250.25867575826792144No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT284750.22646555623917183No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT257130.2044989937691949No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG256930.204339931043127No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT232600.18498995041696703No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA192110.15278770152452079No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT151890.12080018731226623No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT138360.11003959389377282No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG137450.10931585849016387No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC129390.10290563062962751No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA128580.10226142658905253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG69500.018.0529021
GAGTACG11800.016.8592991
TACCTGG72950.016.4987132
GTACGCA10400.016.443
CGCGGTA6450.016.39389443
AGTACGC10250.016.2416232
GTACCCG7100.016.2386781
TCGTGCG1908.021743E-915.3946579
GTACCGG5950.015.197841
CGCCGTA5200.014.70970825
TACCCGG7300.014.4842692
ACCTGGG82800.014.1012823
TAACGGC7500.014.09818936
CCAACCG9900.014.0896643
GTACATG273050.014.0003951
TACGCAG11900.013.9897434
CGGTACC7550.013.70739545
TCGCCCC9800.013.54401838
GCTCCGA11000.013.49837429
GCCGCGG8850.013.47268641
Produced by FastQC (version 0.11.5)