FastQCFastQC Report
Wed 25 May 2016
SRR1700239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13528660
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA162960.12045538878203756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG76150.016.7712171
GTATCAA342600.016.6330361
CGCGGTA9350.016.12145443
TATAACG5650.015.9270352
TACCTGG78400.015.8683772
GGTATCA212450.015.8531491
GTACATG362650.015.183631
CGAACTA10850.014.72188129
TACATGG354850.014.6323612
TCGCCCC17400.014.48084138
TAGACCG2651.6370905E-1114.4319975
CGACCGT6900.014.34629721
GTATTAG48350.014.259011
GCGAACT11600.014.15755728
ATCAACG404400.013.7852153
ACATGGG363700.013.7629063
TCAACGC406600.013.73275854
CAACGCA410750.013.5994885
TATCAAC420500.013.3483622
AACGCAG420000.013.3106896
Produced by FastQC (version 0.11.5)