FastQCFastQC Report
Wed 25 May 2016
SRR1700238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5009023
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA92980.1856250210869465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.10151680277770735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG92100.032.5603372
GTACCTG93100.032.533351
ACCTGGG96950.030.2353463
TAACGCA4400.026.5872484
TATAACG4700.025.8472462
CCTGGGG67450.024.6815514
GTACCCG8000.022.0383131
TACCCGG7800.021.3429992
ATAACGC5200.021.198793
GTACACG5450.019.492841
CTGGGGG32200.018.7939955
CGCGTAT600.00651828518.74760414
GTACCGG5600.018.5670761
TACCGGG5400.018.3306262
TACACGG6000.017.9973412
CGACCGT2251.8189894E-1115.99779721
CTGGGGA36900.015.0589015
GTACATG106550.014.8921491
CCTGGGA38700.014.8816714
GTATAAC11400.014.8706571
Produced by FastQC (version 0.11.5)