FastQCFastQC Report
Wed 25 May 2016
SRR1700237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7274380
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90210.12401056859828603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG117850.030.9647461
TACCTGG117750.030.664232
ACCTGGG124450.028.4529823
CCTGGGG89150.023.7460964
GTACCCG10150.023.1665231
GGTATCA75550.022.8640141
TACCCGG9750.022.6120242
TAACGCA5650.021.5015224
ATAACGC6000.021.3718243
TATAACG5800.021.3330352
GTATCAA138950.020.1444631
GTACCGG8900.019.5611461
GTACACG7850.019.2973981
TACCGGG7850.018.9143852
CTGGGGG46700.016.9088555
TCAACGC169850.016.1194024
ATCAACG168400.016.1110273
TACACGG8800.016.105562
CAACGCA173450.015.81078155
TATCAAC175400.015.5706612
Produced by FastQC (version 0.11.5)