FastQCFastQC Report
Wed 25 May 2016
SRR1700236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6689209
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA360310.5386436572694918No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA170450.254813386754697No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT113830.17016959703307222No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT105430.15761205846610565No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC92230.13787878357515815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90350.1350682868482656No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA74140.11083522730415508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.10356979427612442No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68510.1024186865741525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG101200.031.568491
TACCTGG102250.030.7344342
ACCTGGG106200.028.977023
TATAACG5800.022.8831652
CCTGGGG76150.022.8350354
GTACCGG6500.021.9138831
GTACCCG9800.020.9945931
TAACGCA6500.019.7266644
ATAACGC6300.019.638773
TACCCGG10500.019.0674612
TACCGGG7250.018.3065342
CTGGGGG39350.016.692845
CTGTCGC13400.016.620179
TCTGTCG11500.016.4318528
GTACACG7300.016.4151611
TACACGG7600.014.799552
GTCTCGC10100.014.7745641
TGTCGCC13600.014.05999410
TCTATAC15950.013.3984653
CTGGGGA44350.013.2892295
Produced by FastQC (version 0.11.5)