FastQCFastQC Report
Wed 25 May 2016
SRR1700235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924349
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA203680.2282295324846664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113260.12691121783785014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA95480.10698819600174758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG111500.026.2406081
TACCTGG110400.026.041072
ACCTGGG115850.024.4664823
TATAACG6650.019.6201972
ATAACGC7500.019.196223
CCTGGGG91450.018.8918514
TAACGCA7000.017.3537254
TACCCGG11000.016.3604152
CGCGTAT2101.07320375E-1016.06916412
GTACCCG12550.015.6743661
GTACACG8400.014.266291
GTACCGG9200.013.7630491
GTATTAG23250.013.7123091
GTACATG214250.013.2340011
GTATCAA299450.013.2213641
TTAACGC2053.376408E-713.16909822
CGAACTA4800.013.125829
TATACTG29950.012.9189925
CTGGGGG49300.012.913285
TACACGG8300.012.7384562
Produced by FastQC (version 0.11.5)