FastQCFastQC Report
Wed 25 May 2016
SRR1700234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6752824
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA190460.28204496370703575No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA89020.13182632925128807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84860.125665943611147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG124450.032.061841
TACCTGG123450.031.9259932
ACCTGGG131000.029.5364693
TATAACG6050.026.4000282
CCTGGGG82350.024.5582524
GTACCGG10450.024.2292731
GTACCCG11050.024.1411741
TAACGCA6600.023.8591794
TACCCGG11250.023.395632
ATAACGC7250.022.9612273
TACCGGG10650.021.9677282
GTACACG6700.021.9319481
TACACGG6300.021.0674932
CGACCGT4000.019.68469821
CTGGGGG41650.019.1740885
GTATAAC13000.017.215931
TCGCCGA4600.016.62806917
GACGCTA7300.016.33425940
GTCTCGC9650.016.164431
ACACGGG7800.016.150833
Produced by FastQC (version 0.11.5)