FastQCFastQC Report
Wed 25 May 2016
SRR1700233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8641522
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG126750.029.9318161
TACCTGG126600.029.3373472
ACCTGGG135650.026.8493963
TATAACG5750.022.6917292
CCTGGGG92950.022.1693784
TAACGCA6650.020.9738184
GTACACG8400.020.1869661
ATAACGC7200.019.9965443
TACCCGG11100.019.253432
TACACGG8800.018.9171852
GTACCCG11850.018.8888661
GTATCAA193350.016.4995421
CTGGGGG47800.016.189675
GTACCGG10000.016.0526751
TACCGGG9950.015.1481362
GGTATCA124200.015.0001171
GTACATG195300.014.3783281
CGACCGT3200.014.06031521
CGCGTAT1453.1125037E-513.96383112
ATCAACG225400.013.8729353