FastQCFastQC Report
Wed 25 May 2016
SRR1700232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7106517
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA145360.20454464542897735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74540.10488963862325244No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA71670.10085109203284816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG115500.031.6410851
TACCTGG115250.031.2700162
ACCTGGG122550.028.7648563
TATAACG6000.026.9952752
ATAACGC6250.025.1955913
CCTGGGG83700.024.6193494
TAACGCA6650.022.6652094
GTACCGG9150.022.2440261
GTACCCG10550.020.3640041
TACCCGG10100.020.2687322
TACCGGG8600.020.1418252
CTGGGGG42300.017.7096655
GTACACG7550.017.3729291
TCTGTCG11650.017.186688
TACACGG7850.016.3347232
ACCGTCG1156.797715E-515.6502068
GTATAAC15600.015.5114081
GTCTCGC11900.015.2032011
TACACCG4400.014.3156775
GTACATG152100.013.6788821
Produced by FastQC (version 0.11.5)