FastQCFastQC Report
Wed 25 May 2016
SRR1700231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8103506
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA319360.39410102244633366No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA155940.19243522495078055No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT102570.12657484303707556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97380.12017020780881757No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT94230.11628300145640665No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC81700.10082055841014988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG117150.031.2714331
TACCTGG117050.030.6542992
ACCTGGG122850.028.5112043
TATAACG6650.023.3415432
GTACCGG9200.023.1054841
ATAACGC6950.022.9813563
TAACGCA7200.022.8082794
CCTGGGG86750.022.6125284
GTACCCG9500.022.3758371
TACCCGG10100.020.4912572
GTACACG8550.019.8367371
TACCGGG9550.018.3735622
CTGGGGG44950.017.1658975
TACACGG9000.016.9968532
ACACGGG9950.014.6957723
GTATAAC18500.014.3017511
TAGACCG1656.935001E-613.6338395
GTACATG174900.013.5890141
GTCTCGC10500.013.5683271
GTATTAG23900.013.4358611
Produced by FastQC (version 0.11.5)