FastQCFastQC Report
Wed 25 May 2016
SRR1700230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10172477
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113980.11204743937980888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG88550.023.6454241
TACCTGG88250.023.1724382
GGTATCA127100.020.405281
ACCTGGG99050.020.2369843
GTACCCG10000.019.445681
GTATCAA225900.019.2480951
TACCCGG10000.018.6724222
GTACCGG9000.016.8328251
CGAACTA7150.016.67675629
TACCGGG8200.015.6380842
ATCAACG275200.015.4175653
TCAACGC277850.015.3031344
CAACGCA281800.015.0886285
TAGACCG2405.0931703E-1114.9981515
TATAACG5700.014.9979282
TATCAAC285900.014.8877662
AACGCAG291200.014.6788226
GTACATG256950.014.5901791
ATAACGC7100.014.5754533
TATACTG32750.014.563095
Produced by FastQC (version 0.11.5)