FastQCFastQC Report
Wed 25 May 2016
SRR1700229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9341064
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA617530.661091712892664No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA303360.324759577709777No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT205430.21992141366336854No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT196660.21053276157833842No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC158030.16917772964621589No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA122830.1314946562832671No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT120320.12880759622244317No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA109630.11736350377216129No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT96710.10353210298098803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93670.10027765573600608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG126350.028.6643731
TACCTGG124400.028.5553152
ACCTGGG133750.025.9031243
TATAACG6450.021.2762622
TACCCGG12800.021.0909672
GTACCCG12900.021.030361
ATAACGC6950.020.716693
TAACGCA6750.019.9973624
CCTGGGG96550.019.596074
GTACCGG9750.018.3179841
TAGACCG1853.092282E-1017.024785
TACCGGG9750.016.8439312
TACACGG8350.016.7043822
TACCGTC4000.016.3114837
GTACACG9750.015.9992511
GTATAAC17650.014.3459221
GTACATG189250.014.0603381
CTGGGGG52100.013.9904785
TACACCG6950.013.9190265
TACATGG189600.013.4673682
Produced by FastQC (version 0.11.5)