FastQCFastQC Report
Wed 25 May 2016
SRR1700228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7210122
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA226870.3146548699175964No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA104000.14424166470414787No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT74650.10353500259773692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG110200.030.6989841
TACCTGG109600.030.2151722
ACCTGGG117250.027.4762843
GTACCCG10200.024.814281
CCTGGGG79650.023.6133374
TACCCGG10600.023.3461152
TAACGCA6750.021.664394
GTACACG8650.021.4230631
ATAACGC7100.020.2791373
TATAACG7500.019.1975822
TACACGG9300.018.384782
GTACCGG7800.017.9630851
ACACGGG9650.017.4848463
CTGGGGG42550.017.2896045
TACACCG4950.015.9074185
CGCGTAT1004.1717573E-415.74954612
TACCGGG8450.014.9093662
GTACATG154150.014.7041941
GTATAAC16950.014.6658351
TACATGG153500.014.2897322
Produced by FastQC (version 0.11.5)