FastQCFastQC Report
Wed 25 May 2016
SRR1700227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7014766
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86210.12289789851863911No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA81670.11642583658528309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG110100.031.1103211
TACCTGG109800.030.753942
ACCTGGG115250.028.772593
TATAACG5950.027.223122
CCTGGGG86000.023.4909344
ATAACGC8150.022.0828323
TAACGCA7450.022.0439284
TACCCGG10450.021.0975572
GTACCCG10400.020.6523481
GTACCGG7200.019.7827741
TACCGGG7300.016.9497072
CGCAACG1904.5110937E-1016.57665317
CTGGGGG44050.016.2406585
GTATAAC15550.016.1388191
GTATCAA172500.015.41336251
GGTATCA114450.014.756521
GTACACG7700.014.6810951
CTGGGGA49450.014.1941925
GTACATG153050.014.1813331
ACACCGT6950.013.595246
Produced by FastQC (version 0.11.5)