FastQCFastQC Report
Wed 25 May 2016
SRR1700226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151479
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA195110.46997708527491044No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA93060.2241610760887867No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT62750.15115095126339312No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT54160.130459530205982No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47910.11540465458213808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.11381485971626015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG70550.032.03191
TACCTGG70300.031.6493842
ACCTGGG76200.028.7559873
TATAACG3600.027.4963112
CCTGGGG48850.024.085824
TAACGCA4300.023.5433524
ATAACGC4300.023.0201663
TACACGG3500.022.4969842
GTACACG3500.021.9528351
GTACCCG5350.019.430491
TACCCGG5350.018.9226972
CGTATCG901.8690748E-417.49765415
TACCGGG4200.017.1405582
ACGACCG800.001713398816.8727365
GTACCGG4750.016.651531
CTGGGGG23950.016.6261625
GTATAAC9750.015.5292281
ACACGGG5250.014.9979893
CGACCGT1251.3832617E-414.39824321
TAGACCG950.005383929214.2086215
Produced by FastQC (version 0.11.5)