FastQCFastQC Report
Wed 25 May 2016
SRR1700225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834322
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG100850.027.422561
TACCTGG98800.027.3921662
ACCTGGG107650.024.42973
TAACGCA5350.020.604534
TATAACG5550.019.4565532
ATAACGC5600.018.8811073
CCTGGGG85400.018.8087864
GTATCAA171350.016.4038011
GTACCCG9300.016.0479091
TACCCGG9100.015.8218122
GTACACG6950.015.6175841
GGTATCA111850.015.36508751
GTACATG179600.015.32292651
TACATGG179300.014.5669822
TACACGG6900.014.3456832
TAGACCG2103.0029696E-813.926585
ACATGGG182100.013.8241293
CTGGGGG49650.013.7744665
TCAACGC202450.013.534744
ATCAACG202400.013.4935383
Produced by FastQC (version 0.11.5)