FastQCFastQC Report
Wed 25 May 2016
SRR1700224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7312185
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA1029841.4083888741874009No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA508380.6952504620711867No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT342240.4680406745726483No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT306590.41928643763799744No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC259650.3550922193571416No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA209420.28639866195945535No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT206850.2828839806432687No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA180550.2469166193141995No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT165630.22651232155641576No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG162720.22253266294548074No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT145250.19864103547708378No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT130520.17849657797224772No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA108180.14794483454671895No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT86370.11811790866888626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78080.10678066815869676No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG77060.10538573627445148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG95450.029.4472961
TACCTGG94800.028.997812
ACCTGGG99950.026.6709123
TATAACG5400.022.9124342
CCTGGGG68950.021.8596324
GTACCCG9450.021.7750851
TACCCGG9250.021.4014532
TAACGCA6150.020.8498084
ATAACGC6050.020.8226013
GTACCGG6300.018.6643581
GTACACG7550.017.9702661
TACGCAG10200.016.9821594
GAGTACG12350.016.8450051
AGTACGC10700.016.6090812
GTACGCA10700.016.1885993
ACGCAGT11350.015.45975
GTATAAC14300.015.3386081
TACACGG7550.015.1958692
CTGGGGG35800.014.8296645
CGCGGTA2751.8189894E-1214.72556143
Produced by FastQC (version 0.11.5)