FastQCFastQC Report
Wed 25 May 2016
SRR1700223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491343
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA92200.20528380931939513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.12833577840748303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46170.10279776004638255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG79050.031.7981241
TACCTGG79900.030.886222
ACCTGGG81300.029.607263
CCTGGGG57450.025.6090034
ATAACGC3700.024.9279793
GTACCCG6100.023.345831
TAACGCA4150.022.7670154
TATAACG4200.022.4959812
TACCCGG6900.020.5398082
CTGGGGG30050.019.9132485
GTACACG4750.018.5596431
GTACCGG4500.017.5814291
TACCGGG4650.017.4162432
GTCTCGC7100.016.8739261
TACACCG2900.016.2901945
TATACCG1803.929017E-916.2470975
TACACGG5150.015.7253462
CGACGCC2202.1827873E-1015.34226833
CCGCGTA900.00375981614.99782211
CTGGGGA33200.014.6359395
Produced by FastQC (version 0.11.5)