FastQCFastQC Report
Wed 25 May 2016
SRR1700222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10905468
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA429150.3935181873900322No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA209080.19172033699058122No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT143620.13169540270990662No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT139890.12827510016076338No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC114330.10483731647280062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG75700.020.1587161
TACCTGG76200.019.7205312
CGAACTA13350.018.20359429
CGCGGTA10450.018.08447643
GCGAACT13750.017.83285928
CGTAGCG3550.017.7429915
GTACCCG9200.017.6929241
TATAACG4750.017.049282
ACCTGGG86050.017.0448763
TACCCGG9200.015.8936152
CGGTACC12500.015.29811545
TCGCCCC17950.015.29101638
CCAACCG19100.014.95937143
TAACGCA5350.014.7167344
GTACATG315800.014.7114221
GCTCCGA19850.014.5098929
CGCCCCA20700.014.12908439
TAACGGC13400.014.10468136
GTACCGG6900.014.0888111
TACATGG306500.013.9450572
Produced by FastQC (version 0.11.5)