FastQCFastQC Report
Wed 25 May 2016
SRR1700221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7605371
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG120950.030.3815521
TACCTGG120100.030.1190412
ACCTGGG128200.027.6545313
TATAACG6650.024.3561692
TAACGCA7250.023.8919094
ATAACGC7300.023.7282663
CCTGGGG85150.022.2182314
GTACCGG8450.021.9441931
TACCCGG10100.020.7137972
GTACACG7150.020.5574741
GTACCCG11050.020.4644571
TACACGG7000.020.2460652
GGTATCA88350.019.273071
GTATCAA149650.018.5106561
TACCGGG8650.018.4646192
CGCGTAT1354.2882675E-818.33085412
CTGGGGG45850.016.3381545
GTATAAC15800.015.457141
ATCAACG174650.015.237523
TCAACGC175500.015.1380834
Produced by FastQC (version 0.11.5)